If you use an older Decomb (version 4 that is), you don't need to specify the field order IIRC. However, it's very highly recommended that you use Decomb 5, Don released a new version (v5.1) a while ago. Decomb 5 is better optimized and smarter in every way.
With Decomb 5, you'll specify the field order in Telecide by the "order" argument, like this: Telecide(order=0) or Telecide(order=1). The former means bottom field first and the latter top field first. FieldDeinterlace doesn't need the argument.
EDIT: And what comes to TMPGEnc, the program will show a tooltip if you leave the mouse pointer over the field order window for a while. Mostly it's needed when TMPGEnc deinterlaces or IVTCs the video - but most importantly if you encode as MPEG-2 interlaced, the field order must be correct or else you'll get nasty artifacts and jerky motion.
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