I think I understand you here ... you want an ISO, not a VIDEO_TS folder set, to ensure data integrity. If so, burn a valid DVD ISO in Imgburn
(UDF 1.02, ISO 9660, no Joliet). Playback with something like VLC, or mount the ISO with sometihng like MagicISO.
There's no such an animal as "writeable" ISO, if you mean like RAM drive or zip file. It's an image. Not that I've seen, at least.